|Title:||Spectrophotometric Determination of Cholesterol with Dichromate|
|Publisher:||Addis Ababa University|
|Abstract:||The oxidation of cholesterol by dichromate has been investigated for its application to quantitative determin-ation of cholesterol in blood sera. The redox system was studied spectrophotometrically by measuring the light ab-sorption of the reduction product, chromium(III), at 585 nm in benzene-acetic acid and pertoleum ether-acetic acid. The effects of experiemntal variables have been studied to establish the optimum conditions of the redox reaction. It has been found that consistent and reproducible results are obtained by carrying out the reaction at 800C for 1 hr. It has also been found that petroleum ether can be used as an alternative to benzene as a solvent to introduce cholesterol into the reaction system. The coloured system has been found to obey Beer's law in the concentra-tion range 0.01-2.0mg ml-1 of cholesterol. Other photo-metric characteristics of the coloured system have also been evaluated. On the basis of these studies a simple and precise method has been developed for the determin-ation of cholesterol. The method'has been applied to the analysis of blood sera and the results obtained have been compared with clinical results. The proposed method has been found to give results which are in good agreement with those of routinely used clinical methods.|
|Description:||A Thesis Presented to the School of Graduate Studies Addis Ababa University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Chemistry|
|Appears in Collections:||Thesis - Chemistry|
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