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Title: Analysis of Testicular growth Factors by Quantitative Polymerase Chan Reaction
???metadata.dc.contributor.*???: Dr. Yisehak Worku
Dr. Mikeal Holst
Dr. Olle Soder
Ayele, Dereje
Issue Date: Feb-1996
Publisher: Addis Ababa University
Abstract: Expression of testicular Interieukin-1 was analyzed both at the level of biologically active peptide and its encoding mRNA, using a sensitive bioassay, Thymocyte proliferation assay, and Polymerise Chain Reaction. in order to analyse the relative change in the level of IL-1 a mRNA expression a Quantitative PCR technique was developed involving an internal standard IL-la RNA construct derived from rat macrophage IL-la cDNA preparation. The IL-1 a mRNA expression during postnatal rat development could be detected by PCR as early as 20 days, postnatal, and The intensity of amplified cDNA band was found to increase with increasing days of postnatal age. By using Quantitative PCR, a relative increase in the level of amplified IL-la cDNA could be demonstrated starting from day 20, and this increase in the level of amplified cDNA is found to reach a sustained high level from 30 to 60 postnatal days of rat testicular development. This study shows that the level of IL-1 a mRNA rises in parallel to the level of ibla peptide during maturity, previously demonstrated, and suggests that it is controlled at the level of mRNA expression, probably at transcriptional level. The role of testosterone in the regulation of tIL-1 a was analysed using rats treated with, EDS with and without testosterone replacement, and androgen receptor blocker, cyproterone acetate. The result showed that EDS pretreatment resulted in an increase in the level of both IL-la bioactivity and the level of its mRNA. Exogenous testosterone replacement along with EDS treatment significantly reduced the effect of EDS treatment on the expression of tIL-1 a peptide and mRNA, Cyproterone acetate treatment in contrast resulted in a decrease in the 1L-1 a mRNA levels. This study shows that t1L- is expression is developmentally regulated at the level of its mRNA expression. It also suggests that testosterone regulates testicular IL-1a expression at the level of transcription, This finding further strengthens the importance of testicular IL-la as a paracrine growth factor in the regulation of testicular divelopment and function.
Description: A Thesis Presented to the School of Graduate Studies Addis Ababa University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Chemistry
Appears in Collections:Thesis - Chemistry

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