Skip navigation
 

Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/11312
Title: ISOLATION, IDENTIFICATION, ANTIMICROBIAL PROFILE AND MOLECULAR CHARACTERIZATION OF ENTEROHAEMORRHAGIC E. COLI O157:H7 ISOLATED FROM RUMINANTS SLAUGHTERED AT DEBRE ZEIT ELFORA EXPORT ABATTOIR AND ADDIS ABABA ABATTOIRS ENTERPRISE
???metadata.dc.contributor.*???: Dr. Tesfaye Sisay
Dr. Aklilu Feleke
Asmelash, Tassew
Keywords: E. coli O157:H7;PCR;Ruminants
Issue Date: Oct-2015
Abstract: A cross sectional study was conducted from December 2014 to June 2015 on apparently healthy ruminants slaughtered at Debre Zeit ELFORA export abattoir and Addis Ababa Abattoirs Enterprise in order to determine the prevalence of E. coli with special emphasis on E. coli O157:H7 and to assess its antimicrobial resistance pattern. The study was also aimed to molecularly characterize the shiga toxin producing E. coli strains using multiplex PCR technique. 635 carcass swab samples were collected and processed according to the standard to isolate and identify the pathogen that lead to an overall prevalence of 12.9% (E. coli) and 5.5 % (E. coli O157:H7). The prevalence of E. coli O157:H7 recorded had a statistical difference among animals species and age groups (P<0.05). However, the result showed that the prevalence of E. coli O157:H7 among animals’ origin was not found to be statistically significant (P<0.05). The prevalence of E. coli O157:H7 was higher in the Addis Ababa abattoir enterprise than that of the privately owned abattoir. Furthermore, E. coli isolates were subjected to the in vitro antimicrobial sensitivity test and the result indicated the presence of different degrees of susceptibility and resistance to most of the antibiotics. The degree of susceptibility ranges from 14.03% up to 85.75% and resistance it is between 5.75% and 48.6%. Out of the 35 E. coli O157:H7 isolates, only 5 (5.7%) of them showed the presence of multidrug resistance. Analysis of multiplex PCR assay in detecting the virulence genes revealed that 3 out of 20 (15%) were positive in which only one isolate were found to contain both Stx1 and Stx2 genes. In conclusion, the presence of E. coli O157:H7 in carcasses that developed resistance can be considered as potential pathogens to the society having the habit of eating uncooked meats. Therefore, strict measures like practicing proper hygiene in abattoirs and antibiogram test to select effective drugs should be implemented.
Description: A Thesis submitted to the College of Veterinary Medicine and Agriculture of Addis Ababa University in partial fulfillment of the requirements for the degree of Master of Science in Veterinary Microbiology
URI: http://hdl.handle.net/123456789/11312
Appears in Collections:Thesis - Tropical Veternery Medicine

Files in This Item:
File Description SizeFormat 
Asmelash MSc Thesis December 2015.pdf1.07 MBAdobe PDFView/Open
Show full item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.