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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/536

Title: EVALUATING BRONCHODILATOR , ANTI-RESPIRATORY DISTRESS EFFECTS and LD50 DETERMINATION of HYDROALCOHOLIC EXTRACT OF THE LEAVES of Adhatoda schimperiana in ANIMAL MODEL.
Authors: Hailemeskel, Mekonnen
Advisors: Professor Eyasu Makonnen
Dr. Asfaw Debella
Dr. Yalemstehay Mekonnen
Keywords: Adhatoda schimperiana
guinea-pig
tracheal chain
bronchodilator
respiratory distress
LD50
phytochemical screening.
Copyright: 2005
Date Added: 16-Apr-2008
Publisher: Addis Ababa University
Abstract: In the present study relaxant effect of hydroalcoholic extract of the leaf of Adhatoda schimperiana was tested on isolated guinea-pig tracheal chains in vitro; and its antirespiratory distress effect in guinea-pig in vivo. The bronchodilatory effect of the hydroalcoholic extract was examined on pre-contracted isolated guinea-pig tracheal chains using acetylcholine and histamine as standard spasmogens. Two different concentrations of the extract (400 and 800 μg/ml), and atropine and diphenhydramine as standard antispasmogens were used. The oral LD50 of the extract was evaluated using mice and major chemical constituents were identified using chemical test and TLC techniques. The hydroalcoholic extract showed a statistically significant (P<0.05 to p<0.001), dose dependent relaxant effect as evidenced by a clear rightward shift in the cumulative doseresponse curves of acetylcholine and histamine. Tissue contact time variation had a significant effect on response to histamine induced contraction (p<0.05) but not to that of acetylcholine. The maximum response obtained in the presence of the hydro-alcoholic extract was significantly inhibited (P<0.05 to p<0.001) as compared to the standard spasmogens. The EC50 obtained in the presence of the extract was not significantly different than that of the standard spasmogens. In the in vivo experiments, the extract showed protective effect against histamine induced respiratory distress, but the observed effect was lower than that of diphenhydramine. The oral LD50 of the extract was 16.22 g/kg. Phytochemical examination of the hydroalcholic extract used for pharmacological evaluation revealed the presence of alkaloids, saponins, phytosterols, poyphenols and glycosides as a major class of compounds. The results suggested that the studied plant leaves extract possesses bronchodilator and anti-respiratory distress effects. The promising finding could help in initiating activityix guided fractionations of the crude hydroalcholic extract to determine the semi purified extract and/or active major components.
Description: A Thesis submitted to School of Graduate Studies of Addis Ababa University in partial fulfillment of the requirements for the degree of Master of Science in Pharmacology. Department of Pharmacology, Faculty of Medicine, AAU.
URI: http://hdl.handle.net/123456789/538
Appears in:Thesis - Pharmacology
Thesis - Pharmacology
Thesis - Pharmacology

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