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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/49

Title: IN VITRO EVALUATION OF ANTI MICROBIAL ACTIVITIES OF ALBIZIA GUMMIFERA AND CROTON MACROSTACHYUS AGAINST CLINICAL ISOLATES OF NEISSERIA GONORRHOEAE
Authors: MESFIN, TEFERA
Keywords: Antibacterial activity
Albizia gummifera
Croton macrostachyus
Neisseria
Date Added: 3-Sep-2007
Abstract: A total of 250 urogenital specimens were collected from male and female adult patients who were clinically symptomatic for uncomplicated gonococcal infection from eight health centers in Addis Ababa over a period of six months (May 2005-October 2005). Among the 250 urogenital specimens collected from both sexes, the etiologic agents were isolated from 19 patients, indicating an 8% prevalence of N. gonorrhoeae infection. The agar dilution method was used to evaluate in vitro anti bacterial effects of crude and solvent fractions of two traditionally claimed medicinal plants namely, Croton macrostachyus and Albizia gummifera against clinical isolates and reference strain of N. gonorrhoeae (ATCC 49226). Crude hydro-alcoholic (20-80%) extracts of both plants were effective against the test organisms and their minimum inhibitory concentrations (MICs) were between 250-500 μg/ml. Solvent-solvent partition method was then used to divide the crude extracts of these plants into three fractions and determined their anti N. gonorrhoeae activity. Chloroform and n-butanol fractions were identified to be the more active ones in C. macrostachyus with MIC values between 125-250 μg/ml. The most active fraction in A. gummifera was identified to be the n- butanol fraction, which also had MIC values between 125-250 μg/ml. Aqueous fractions of this plant exhibited activity at MIC values of 500-1000 μg/ml. However, aqueous fraction of C. macrostachyus had no growth inhibition effect. MIC values for Penicillin and Spectinomycin were 128 and 256 μg/ml respectively. With this aspect, these results indicate the presence of chemical compounds in A. gummifera and C. macrostachyus with anti N. gonorrhoeae activity comparable to Penicillin or Spectinomycin and the need for further investigation. The results also substantiate the ethno-botanical use of these medicinal plants for the treatment of gonococcal infections.
Description: I, the undersigned declare that this MSc thesis is my original work and it has not been presented in any other institution/university for a similar degree or other purpose. I also declare that all sources of materials used for this study have been fully acknowledged.
URI: http://hdl.handle.net/123456789/49
Appears in:Thesis - Biology

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