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http://hdl.handle.net/123456789/43
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| Title: | A Study on the Response of Three Genotypes of Mucuna pruriens to Different Growth Media for Tissue Culture Differentiation |
| Authors: | Hanna, Beksissa |
| Keywords: | Fabaceae
Mucuna pruriens
meristem culture
tissue culture |
| Date Added: | 31-Aug-2007 |
| Abstract: | In vitro regeneration using meristem explants to induce shoots and roots in Mucuna pruriens
genotypes were investigated. Different treatment combinations of culture media containing
growth hormones, nutrients and vitamins were evaluated to promote shoots and roots directly
from the meristem explants and indirectly via callus phase. A total of 13 experiments were
conducted. Among the various cytokinins, BAP, Kn, 2iP, GA3 and TDZ tested, the survival
of tissue (shoots and callus) was found to be effective in BAP combined with an auxin NAA.
The cytokinins, BAP and TDZ induced multiple shoots. An optimal level of 0.1mg/l BAP
supplemented with liquid media containing ¼ G-B5 + 7.5 g/l sucrose supplemented with
5mg/l IBA was effective in root induction. BAP along with an auxin NAA was effective for
survival of tissues (shoots as well as callus). Rooting was induced at the base of shoots
proliferated on media containing lower concentrations of BAP. However, shoots proliferated
in TDZ containing media were difficult to root. No organogenesis was observed from callus
regardless of the different media evaluated. Among the accessions, 10084, 14880 and 15169,
accession 14880 performed relatively better in tissue survival percentage as well as percent
of shoot survival. Results from callus and shoots were often different and should not be
compared. |
| Description: | A Thesis Submitted to the School of Graduate Studies of Addis
Ababa University in Partial Fulfillment of the Requirements for
the Degree of Master of Science in Biology |
| URI: | http://hdl.handle.net/123456789/43 |
| Appears in: | Thesis - Biology
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