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Title: A Study on the Response of Three Genotypes of Mucuna pruriens to Different Growth Media for Tissue Culture Differentiation
Authors: Hanna, Beksissa
Keywords: Fabaceae
Mucuna pruriens
meristem culture
tissue culture
Date Added: 31-Aug-2007
Abstract: In vitro regeneration using meristem explants to induce shoots and roots in Mucuna pruriens genotypes were investigated. Different treatment combinations of culture media containing growth hormones, nutrients and vitamins were evaluated to promote shoots and roots directly from the meristem explants and indirectly via callus phase. A total of 13 experiments were conducted. Among the various cytokinins, BAP, Kn, 2iP, GA3 and TDZ tested, the survival of tissue (shoots and callus) was found to be effective in BAP combined with an auxin NAA. The cytokinins, BAP and TDZ induced multiple shoots. An optimal level of 0.1mg/l BAP supplemented with liquid media containing ¼ G-B5 + 7.5 g/l sucrose supplemented with 5mg/l IBA was effective in root induction. BAP along with an auxin NAA was effective for survival of tissues (shoots as well as callus). Rooting was induced at the base of shoots proliferated on media containing lower concentrations of BAP. However, shoots proliferated in TDZ containing media were difficult to root. No organogenesis was observed from callus regardless of the different media evaluated. Among the accessions, 10084, 14880 and 15169, accession 14880 performed relatively better in tissue survival percentage as well as percent of shoot survival. Results from callus and shoots were often different and should not be compared.
Description: A Thesis Submitted to the School of Graduate Studies of Addis Ababa University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Biology
URI: http://hdl.handle.net/123456789/43
Appears in:Thesis - Biology

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