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Title: Level of Essential and Non-Essential Metals in Gesho (Rhamnus prinoides) Cultivated in Ethiopia
Authors: Afewerk, Gebre
Advisors: B.S. Chandravanshi(Prof.)
Keywords: Rhamnus prinoides
Gesho
Leaf
Stem
Stem
Minerals, Flame atomic absorption spectroscopy
Local alcoholic beverages
Medicinal uses
Wet digestion
Copyright: Jun-2011
Date Added: 17-Jul-2012
Publisher: Addis Ababa University
Abstract: Rhamnus prinoides leaves and stems have been used for bitterness of local alcoholic beverages in Ethiopia for several hundreds of years and as traditional medicine in some African countries like Kenya and South Africa; never the less, level of essential and non-essential elements of this plant has not been conducted so far. Thus, this study has assessed the level of essential (Mg, Ca, Cr, Mn, Fe, Co, Cu and Zn) and non-essential elements (Cd and Pb) of R. prinoides leaves and stems cultivated in Ethiopia using flame atomic absorption spectroscopy. The solutions for the analysis of the metals in leaf and stem mentioned above were prepared by the carefully optimized mixture of HNO3, H2O2 and HClO4 reagents using wet digestion with a reflux condenser. The efficiency of the optimized procedure was validated by spiking experiment and the percent of recovery for all the metals was (92-103 for leaf samples and 91-103 for the stem samples). The concentrations of all the metals mentioned above were analyzed and the concentrations were reported in (mg/kg) except the concentration of chromium at Simche zala in stem sample, the concentrations of cadmium in stem samples at two sample sites (Kesatyebirhan ,Gedga Amba and Migra) and the concentrations of lead in stem at all sample sites were below the method detection limit and hence were not reported. The concentrations of the metals found were: Mg (3202–4622), Ca (6304– 22236), Cd (0.81–3.10), Cr (5.10–18.7), Mn (8.12–20.6), Fe (47.9–187), Co (22.2–42.1), Ni (12.8– 27.3), Cu (6.5–73.0), Zn (12.2–43), Pb (17.7–25.0) in leaf samples and Mg (2635–5528), Ca (3601–5675), Cd (1.00–1.56), Cr (5.42–16.3), Mn (2.16–3.82), Fe (22.0–124), Co (18.7–91.7), Ni (9.68–19.2), Cu (16.6–233) and Zn (17.4–28.2) in stem samples.
Description: A thesis submitted to school of graduate studies of Addis Ababa University in partial fulfillment of the requirements for the Degree Of Master of Science in Chemistry
URI: http://hdl.handle.net/123456789/3392
Appears in:Thesis - Chemistry

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