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Title: In vitro regeneration of two grapevine (Vitis vinifera L.) varieties from leaf explants.
Authors: Fikadu, Kumssa
Advisors: Tileye Feyissa(Dr.)
Keywords: Organogenesis
growth regulators
callus induction
Copyright: Jul-2011
Date Added: 4-Jul-2012
Publisher: Addis Ababa University
Abstract: Grapevine (Vitis vinifera L.) is one of the most widely distributed fruit crops in the world. It belongs to the family Vitaceae. Conventional method of grapevine propagation is time consuming and allows disease transmission. A planted grape vine needs four to five years to be a propagation material by cutting, due to its long juvenility period. Therefore, the establishment of efficient in vitro regeneration to is much needed. The objective of this study was to develop protocol for the regeneration of grapevine plants from leaf explants of varieties canonannon and chenin blanc. Many factors influence the grapevine in vitro approach. The factor considered under this study was the effect of different hormone concentrations and combinations on two grape varieties (chenin blanc and canonannon). MS medium supplemented with 42 different combinations of auxins (NAA or IBA), and cytokinins (BAP or TDZ) was used for regeneration of shoots from leaf. From the tested six different concentrations of TDZ and eight different concentrations of BAP, the best direct shoot regeneration was obtained at 2mg/l BAP for both chenin blanc (2.3 ± 0.3) and canonannon (2.2 ± 0.2). Among sixteen different combinations of TDZ and NAA, the maximum and best shoots per explant (1.5 ± 0.2) were obtained at 1mg/l TDZ and 0.1mg/l NAA for canonannon. Both varieties did not show any regeneration of shoots at low (0.5mg/l and 1mg/l) and high (4mg/l and 5mg/l) concentrations of BAP. Therefore, frequency of shoot regeneration was greatly influenced by the concentrations of growth regulators. Efficient rooting of in vitro regenerated plants and subsequent establishment was achieved on a medium supplemented with 2mg/l IBA for both varieties of grapevines. The survival rate of chenin blanc and canonannon cultivars were 83.3% and 75 %, respectively.
Description: A Thesis Submitted to the School of Graduate Studies of Addis Ababa University in Partial Fulfillment of the Requirements for the Degree of Master of Science in Biotechnology
URI: http://hdl.handle.net/123456789/3256
Appears in:Thesis - Biology

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