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|Title: ||Ethanolic extracts of Warburgia ugandensis against some test microorganisms.|
|Authors: ||Solomon, Mekonnen|
|Advisors: ||Fassil Assefa (Ph.D.) Sisay Feleke (Ph.D.)|
|Keywords: ||ntimicrobial activity|
leaf and heartwood extracts
|Copyright: ||Apr-2010 |
|Date Added: ||10-May-2012 |
|Abstract: ||Warburgia ugandensis Sprague (Family: Canellaceae) is a tree that is
highly utilized in traditional medicine in tropical and warm substropical
countries of Africa. Crude ethanol extracts of leaves and heartwood of W.
ugandensis were tested for in-vitro antimicrobial activity against six
bacterial and a fungi test organisms using the disk diffusion method, agar
well diffusion and broth dilution for minimum inhibitory concentration. E.
coli was found to be the most susceptible bacterial isolate in the Agar well
diffusion and Broth dilution. Meanwhile Shigella boydii was the most
resistant bacterial isolate showing MIC value of 10mg/ml for the leaf
extract. Bacillus subtilis was the second resistant bacterial isolate giving
0.0mm and 11.0mm inhibition diameter at disk diffusion assay and agar
well diffusion, respectively. Streptococcus pneumoniae gave 2.5mg/ml and
5mg/ml MIC value for heartwood and leaf extracts, respectively. The same
Minimum Inhibitory Concentration (MIC) value was recorded for B. subtilis.
(16.7mm) in the agar well diffusion assay for the heartwood extract.
Staphylococcus aureus and Pseudomonas aeruginosa showed the same
MIC value of 2.5mg/ml in leaf and heartwood extract. Candida albicans
showed the highest inhibition diameter of 10.0mm and 30.7mm in disk
diffusion and agar well diffusion, respectively at the highest test
concentration (25mg/ml). In the broth dilution, a MIC value of 1mg/ml
concentration of the leaf and heartwood extracts of W. ugandensis
inhibited the growth of Candida albicans. It can be concluded that W.
ugandensis has the potential as an antimicrobial agent in the future
especially against C. albicans and E. coli.|
|Appears in:||Thesis - Biology|
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