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|Title: ||WOUND HEALING ACTIVITY GUIDED ISOLATION OF ACTIVE CONSTITUENT (S) FROM THE LEAVES OF ALLOPHYLUS ABYSSINICUS (HOCHST.) RADLK.|
|Authors: ||ADEM, YESUF|
|Advisors: ||Dr. Kaleab Asres|
|Keywords: ||ALLOPHYLUS ABYSSINICUS|
|Copyright: ||Oct-2011 |
|Date Added: ||4-May-2012 |
|Abstract: ||The leaves of Allophylus abyssinicus (Hochst.) Radlk. (family: Sapindaceae) are
traditionally used for the treatment of wounds, burns and in arresting bleeding and in skin
diseases in the Ethiopian folk medicine. However, there is no prior scientific study
regarding phytoconstituents and biological activities. Therefore; the present study was
aimed at evaluation of wound healing activity of the plant.
In the present study, the hydro-alcoholic extract, the different solvent fractions and
isolated bands from the methanolic fraction of the leaves of A. abyssinicus were studied
for wound healing activity by incorporating in simple ointment base B.P. at different
concentration. Wound healing activity was studied in rodents viz. excision, incision and
dead space wound models. In case of the excision wound model, wound contraction and
period of epithelization were studied while incision wound model was evaluated by
determining tensile strength. The content of hydroxyproline was determined in case of
dead space wound model. To determine the healing mechanism; antimicrobial, antiinflammatory
(carageenan induced paw oedema) and antioxidant (DPPH scavenging)
activities were studied.
Treatment of wounds with ointment containing 5% and 10% (w/w) of the hydroalcoholic
extract exhibited significant (P < 0.001) wound healing activity in excision wound model.
The methanolic fraction was found to be the most active in all the test models after
fractionation using chloroform, acetone, methanol and water at 200mg/kg. Further
preparative TLC done on the methanolic fraction gave three bands and band three was
found to be the most active in excision (2%), incision and dead space wound models at
25 and 50 mg/kg. It also showed the highest antiinflammatory, antimicrobial and
antioxidant activity. Its structure tentatively deduced to be 2-(2,3,4,6,8,8a-hexahydrooxopyrano[
3,4-b] pyran-4yl oxy) propanoic acid.
In conclusion, the leaves of A. abyssinicus were shown to have promising wound healing
activity. This could probably be due its antimicrobial, antiinflammatory and antioxidant
|Appears in:||Thesis - Pharmacognosy|
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