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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/2597

Title: WOUND HEALING ACTIVITY GUIDED ISOLATION OF ACTIVE CONSTITUENT (S) FROM THE LEAVES OF ALLOPHYLUS ABYSSINICUS (HOCHST.) RADLK.
Authors: ADEM, YESUF
Advisors: Dr. Kaleab Asres
Keywords: ALLOPHYLUS ABYSSINICUS
HOCHST.
Copyright: Oct-2011
Date Added: 4-May-2012
Publisher: AAU
Abstract: The leaves of Allophylus abyssinicus (Hochst.) Radlk. (family: Sapindaceae) are traditionally used for the treatment of wounds, burns and in arresting bleeding and in skin diseases in the Ethiopian folk medicine. However, there is no prior scientific study regarding phytoconstituents and biological activities. Therefore; the present study was aimed at evaluation of wound healing activity of the plant. In the present study, the hydro-alcoholic extract, the different solvent fractions and isolated bands from the methanolic fraction of the leaves of A. abyssinicus were studied for wound healing activity by incorporating in simple ointment base B.P. at different concentration. Wound healing activity was studied in rodents viz. excision, incision and dead space wound models. In case of the excision wound model, wound contraction and period of epithelization were studied while incision wound model was evaluated by determining tensile strength. The content of hydroxyproline was determined in case of dead space wound model. To determine the healing mechanism; antimicrobial, antiinflammatory (carageenan induced paw oedema) and antioxidant (DPPH scavenging) activities were studied. Treatment of wounds with ointment containing 5% and 10% (w/w) of the hydroalcoholic extract exhibited significant (P < 0.001) wound healing activity in excision wound model. The methanolic fraction was found to be the most active in all the test models after fractionation using chloroform, acetone, methanol and water at 200mg/kg. Further preparative TLC done on the methanolic fraction gave three bands and band three was found to be the most active in excision (2%), incision and dead space wound models at 25 and 50 mg/kg. It also showed the highest antiinflammatory, antimicrobial and antioxidant activity. Its structure tentatively deduced to be 2-(2,3,4,6,8,8a-hexahydrooxopyrano[ 3,4-b] pyran-4yl oxy) propanoic acid. In conclusion, the leaves of A. abyssinicus were shown to have promising wound healing activity. This could probably be due its antimicrobial, antiinflammatory and antioxidant effect.
URI: http://hdl.handle.net/123456789/2597
Appears in:Thesis - Pharmacognosy

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