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Title: Determination, enumeration and viability test of Giardia cyst and Cryptosporidium oocyst from municipal drinking water in Addis Ababa
Authors: Tesfalem, Atnafu W/Gabriel
Advisors: Dr. Seyum Leta
Keywords: Fluorescein and PI
health concern
drinking water
Cryptosporidium oocyst
Giardia cyst
Copyright: Mar-2010
Date Added: 4-May-2012
Publisher: AAU
Abstract: Giardia cysts and Cryptosporidium oocysts are (protozoan single cell) causative agent for most of enteric diarrhea and resistant to most of disinfectants. The main purpose of this research was to determine, enumerate and test viability of Giardia cyst and Cryptosporidium oocysts in Addis Ababa municipal drinking water. A total of 115 samples were collected from different sampling sites at source, reservoir, and public tap. A 100 liters of water collected from each sample and a total of over 10,000 liters of water filtered, concentrated and examined for identifying (oo)cysts. Detection of (oo)cysts in water sample was conducted using (USEPA Method 1623) Aqua-GloTM G/C Direct Comprehensive Kit (Waterborne TM, Inc.) fluorescein-labeled monoclonal antibody for Simultaneous Direct Immunofluorescence detection of Giardia cysts and Cryptosporidium oocysts. Giardia detected at source 33.3%, reservoirs 5.88%, and public tap 12.5%. Likewise Crypto was found at source, reservoirs, and public tap 55.6%, 8.82%, and 18.06% respectively. The mean concentration of Giardia and Crypto ranges from 5 to 0.15 and 9.89 to 0.5 respectively. The viability test demonstrated all the (oo)cysts collected was nonviable except at source (untreated water) (DAP-PI+). This can be due to the age of the (oo)cysts, long distance source of contamination, sporulation of the (oo)cyst before maturation with the combination of the disinfectants used in treatment results the non viability (or death) of (oo)cysts. The physico-chemical parameters (temperature and free chlorine residue) significantly correlated with the occurrence of (oo)cyst (p<0.05). In addition occurrence of Giardia cysts and Crypto oocysts was significantly correlated (r=0.55, p<0.05). Giardia cysts and Cryptosporidium oocyst were identified from the Addis Ababa municipal drinking water. Continuous monitoring of Giardia cyst and Cryptosporidium oocysts was necessary for public health concern.
URI: http://hdl.handle.net/123456789/2548
Appears in:Thesis - Environmental Sciences

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