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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/2329

Title: ISOLATION OF BIOACTIVE COMPOUNDS FROM A FOREST LITTER DECOMPOSING MICROFUNGUS
Authors: DAWIT, TSEGAW
Advisors: Dr. Dawit Abate
Keywords: minimal inhibitory concentration
fractionation
bioactive metabolite
solid substrate
taxonomic studies
LDFs
Copyright: Aug-2011
Date Added: 3-May-2012
Publisher: AAU
Abstract: In a screening of fungi isolates associated with litter decomposition (litotrophic) for bioactive metabolite production, LDMFS JP4 was isolated. It was identified to genus level using morphological (cultural) and microscopic characterization to belong to Geotrichum sp. A bioactive metabolite effective against bacteria and fungi, was isolated from this fungus at stationary phase of mycelium culture growing in solid substrate, rice. Through, bioassay guided fractionation of the crude extract using chromatographic methods, the active compound was isolated in pureform. The compound was found to exhibit activity. It was effective against gram positive (S. pnumoneae, S. aureus and B. subtilis) and gram negative bacteria (P. aeruginosa, E. coli and S. boydii) and the yeast, Candidia albicans. The active compound exhibited potent in- vitro antibacterial with a minimal inhibitory concentration (MIC) of 65-75μg/uL against Gram- positive bacteria and 75-80ug/uL against Gram-negative. It was less effective for the yeast, Candidia albicans. The pure compound is promising and further work on chemical characterization is recommended.
URI: http://hdl.handle.net/123456789/2329
Appears in:Thesis - Biology

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