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|Title: ||PREVALENCE AND ANTIMICROBIAL PROFILE OF LISTERIA MONOCYTOGENES IN RETAIL MEAT AND DAIRY PRODUCTS IN ADDIS ABABA AND ITS SURROUNDING TOWNS, ETHIOPIA|
|Authors: ||FIREHIWOT, ABERA|
|Advisors: ||Prof. DP Monga,|
Dr Abebe Mache
|Copyright: ||Mar-2007 |
|Date Added: ||3-May-2012 |
Listeriosis is a disease of humans and animals, which is mostly caused by Listeria
monocytogenes, as the result of food and environmental contamination as well as
zoontic infections. In the world, this disease is becoming an emerging bacterial
disease, with low incidence but high case fatality rate. The objective of this study was
to isolate L. monocytogenes from retail meat and diary products (raw milk, cottage
cheese, cream cake) and to detect their antimicrobial profile.
Two hundred and forty food samples were purchased from food vendors, shops and
supermarkets, using cross-sectional study design, from July to December 2006. L.
monocytogenes isolation were performed according to the standard procedures using
Listeria enrichment broth, Modified Fraser broth, Polymyxin Acriflavine Lithium
Chloride Ceftazidime Aesculine Mannitol (PALCAM) and Oxford Agar (OXA) media
as well as confirmatory broths like rhamnose, xylose, mannitol; blood agar and
Christe Atkins Munch Peterson (CAMP) test. The antimicrobial profile of L.
monocytogenes was also assessed by using the standard disk diffusion method (Kirby
Bauer, Muller Hinton agar) and it was tested against 14 antimicrobial drugs.
Out of 240 food samples tested, 66(27.5%) were positive for Listeria species. The
prevalence rate of L. monocytogenes was found to be 13 (5.4%). The antimicrobial
profile of L. monocytogenes was also assesed and it was found that L. monocytogenes
was sensitive to most drugs except clindamycin which showed the highest resistance
rate (100%) and also to certain extent, to chloramphenicol (53.9%), tetracycline
(31.8%) penicillin (23.1%), and rifampicin (15.4%).
It was shown that L. monocytogenes is prevalent in Addis Ababa and other small
towns which indicated that it might also be present in other towns of the country with
similar prevalence, which requires a surveillance system.|
|Appears in:||Thesis - Medical Microbiology|
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