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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/2286

Title: PREVALENCE AND ANTIMICROBIAL PROFILE OF LISTERIA MONOCYTOGENES IN RETAIL MEAT AND DAIRY PRODUCTS IN ADDIS ABABA AND ITS SURROUNDING TOWNS, ETHIOPIA
Authors: FIREHIWOT, ABERA
Advisors: Prof. DP Monga,
Dr Abebe Mache
Keywords: Listeriosis,
Prevalence
Antimicrobial susceptibility
Copyright: Mar-2007
Date Added: 3-May-2012
Abstract: ABSTRACT Listeriosis is a disease of humans and animals, which is mostly caused by Listeria monocytogenes, as the result of food and environmental contamination as well as zoontic infections. In the world, this disease is becoming an emerging bacterial disease, with low incidence but high case fatality rate. The objective of this study was to isolate L. monocytogenes from retail meat and diary products (raw milk, cottage cheese, cream cake) and to detect their antimicrobial profile. Two hundred and forty food samples were purchased from food vendors, shops and supermarkets, using cross-sectional study design, from July to December 2006. L. monocytogenes isolation were performed according to the standard procedures using Listeria enrichment broth, Modified Fraser broth, Polymyxin Acriflavine Lithium Chloride Ceftazidime Aesculine Mannitol (PALCAM) and Oxford Agar (OXA) media as well as confirmatory broths like rhamnose, xylose, mannitol; blood agar and Christe Atkins Munch Peterson (CAMP) test. The antimicrobial profile of L. monocytogenes was also assessed by using the standard disk diffusion method (Kirby Bauer, Muller Hinton agar) and it was tested against 14 antimicrobial drugs. Out of 240 food samples tested, 66(27.5%) were positive for Listeria species. The prevalence rate of L. monocytogenes was found to be 13 (5.4%). The antimicrobial profile of L. monocytogenes was also assesed and it was found that L. monocytogenes was sensitive to most drugs except clindamycin which showed the highest resistance rate (100%) and also to certain extent, to chloramphenicol (53.9%), tetracycline (31.8%) penicillin (23.1%), and rifampicin (15.4%). 12 It was shown that L. monocytogenes is prevalent in Addis Ababa and other small towns which indicated that it might also be present in other towns of the country with similar prevalence, which requires a surveillance system.
URI: http://hdl.handle.net/123456789/2286
Appears in:Thesis - Medical Microbiology

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