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Addis Ababa University Libraries Electronic Thesis and Dissertations: AAU-ETD! >
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Thesis - Pharmacology >
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http://hdl.handle.net/123456789/2188
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| Title: | INVESTIGATION OF ANTIHYPERGLYCEMIC AND HYPOGLYCEMIC ACTIVITY OF AJUGA REMOTA AND SYZYGIUM GUINEENSE ON MICE |
| Authors: | TEMESGEN, WORKU |
| Advisors: | Professor Hassan Helaly |
| Keywords: | Alloxan
hypoglycemia
hyperglycemia
diabetes
A.remota, and S.gineense |
| Copyright: | Feb-2009 |
| Date Added: | 3-May-2012 |
| Abstract: | ABSTRACT
The leaves of Ajuga remota and Syzygium guineense are traditionally used in Ethiopia for
management of diabetes mellitus. The aim of this study was to evaluate antidiabetic
effect of the aqueous and hydro alcoholic crude extracts of A.Remota and S.guineense on
alloxan induced diabetic and normoglycemic mice and to carry out acute toxicity test of
the extract with better activity. The results showed that hydro alcoholic extract of
A.remota reduced significantly (p<0.05) the blood glucose level of alloxan induced
diabetic mice at doses of 250 and 500mg/kg, one to two hours after treatment. After three
hours of treatment the blood glucose level, however started to rise. At a dose of
1000mg/kg the extract did not significantly reduce the blood glucose level. The used
three dose levels 250,500 and 1000mg/kg produced no effect on normoglycemic mice.
The aqueous extract of A.remota did not reduce the blood sugar level of both diabetic and
normoglycemic mice.
Results of the present study on the hydro alcoholic extract of S.guineense did not show
any significant reduction in blood glucose level of both normoglycemic and alloxan
induced hyperglycemic mice. However, the aqueous extract reduced the blood glucose
level of both normoglycemic and diabetic mice at all doses tested i.e. 50,100 and
200mg/kg in time dependent manner starting from half an hour after treatment. The
aqueous extract of S.guineense was found to be more active; it was fractionated by using
buthanol and dichloromethane and tested for antidiabetic action. The buthanol fraction
and the aqueous residue were found active in both diabetic and normoglycemic mice.
Dichloromethane fraction did not, however show significant reduction in both
normoglycemic and diabetic mice. Phytochemical screening of the aqueous crude extract
of S.gineense revealed the presence of secondary metabolites including Chromophores,
polyphenols, Saponins, phytosteroids, flavonoids and alkaloids which might be accounted
to the antidiabetic activity. Acute toxicity study showed that the non toxic nature of the
aqueous extract of S. gineense up to 30 times its maximum effective dose and its LD50
was found >6000mg/kg. |
| URI: | http://hdl.handle.net/123456789/2188 |
| Appears in: | Thesis - Pharmacology
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