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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/2188

Advisors: Professor Hassan Helaly
Keywords: Alloxan
A.remota, and S.gineense
Copyright: Feb-2009
Date Added: 3-May-2012
Abstract: ABSTRACT The leaves of Ajuga remota and Syzygium guineense are traditionally used in Ethiopia for management of diabetes mellitus. The aim of this study was to evaluate antidiabetic effect of the aqueous and hydro alcoholic crude extracts of A.Remota and S.guineense on alloxan induced diabetic and normoglycemic mice and to carry out acute toxicity test of the extract with better activity. The results showed that hydro alcoholic extract of A.remota reduced significantly (p<0.05) the blood glucose level of alloxan induced diabetic mice at doses of 250 and 500mg/kg, one to two hours after treatment. After three hours of treatment the blood glucose level, however started to rise. At a dose of 1000mg/kg the extract did not significantly reduce the blood glucose level. The used three dose levels 250,500 and 1000mg/kg produced no effect on normoglycemic mice. The aqueous extract of A.remota did not reduce the blood sugar level of both diabetic and normoglycemic mice. Results of the present study on the hydro alcoholic extract of S.guineense did not show any significant reduction in blood glucose level of both normoglycemic and alloxan induced hyperglycemic mice. However, the aqueous extract reduced the blood glucose level of both normoglycemic and diabetic mice at all doses tested i.e. 50,100 and 200mg/kg in time dependent manner starting from half an hour after treatment. The aqueous extract of S.guineense was found to be more active; it was fractionated by using buthanol and dichloromethane and tested for antidiabetic action. The buthanol fraction and the aqueous residue were found active in both diabetic and normoglycemic mice. Dichloromethane fraction did not, however show significant reduction in both normoglycemic and diabetic mice. Phytochemical screening of the aqueous crude extract of S.gineense revealed the presence of secondary metabolites including Chromophores, polyphenols, Saponins, phytosteroids, flavonoids and alkaloids which might be accounted to the antidiabetic activity. Acute toxicity study showed that the non toxic nature of the aqueous extract of S. gineense up to 30 times its maximum effective dose and its LD50 was found >6000mg/kg.
URI: http://hdl.handle.net/123456789/2188
Appears in:Thesis - Pharmacology

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