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|Title: ||LONGITUDINAL BACTERIOLOGY OF BURN PATIENTS AT YEKATIT 12 HOSPITAL BURN CENTER, ADDIS ABABA, ETHIOPIA|
|Authors: ||AWOKE, DERIBIE|
|Advisors: ||Mr. Tamrat Abebe,|
Dr. Adane Mihret
Dr. Yohanes Demisie
Time related bacterial colonization
|Copyright: ||Apr-2011 |
|Date Added: ||3-May-2012 |
Introduction: Burn is one of the most common, devastating and a very painful form of trauma.
Significant burn injuries induce a state of immunosuppression that predisposes burn patients to
infectious complications. Sepsis and the resultant multi-organ failure are the leading causes of
death in intensive burn care units. Rapidly emerging nosocomial and community acquired
pathogens and the problem of multidrug resistance necessitates periodic review of isolation
outline and their antibiotic susceptibility pattern in burn centers.
Objective: The aim of this study was to determine longitudinal bacteriology of burn patients at
Yekatit 12 hospital burn center, Addis Ababa.
Methods and materials: A prospective hospital based study was carried out from Dec 2010 to
Feb 2011. Wound swab and blood samples were collected on 0, 7th, and 14th, days of hospital
stay and processed by conventional culture and biochemical identification procedures. Once
identified, isolates were tested against commonly used antibiotics in the hospital by Modified
Kirby-Bauer disc diffusion method. Data was analyzed by SPSS version 17.0 for Windows.
Results: Forty one patients were enrolled in the study from both burn unit (BU) and outpatient
(OPD). The mean total burned surface area (TBSA) was 11.9%. From 104 pus cultures 101
isolates were identified during the study period. In the first pus culture S. aureus was
predominated, 15(46.9%) but later on Pseudomonas spp was increasing. Both spp together
accounted 87.1% of the total isolates. Regarding blood culture isolates there was no significant
change on time. Of 92 blood cultures 15 gram positive isolates were recognized. Coagulase
negative Staphylococci (CoNS) noted with the largest percentage, 8 (53.3%) followed by S.
aureus 6 (40%). Staphylococcus aureus showed moderate degree of resistance to the commonly
used antibiotics. Majority of isolates were susceptible for clindamycin and vancomycin. But
Pseudomonas spp were resistant for most of antibiotics used in the hospital. However, it was
found more sensitive to norfloxacine as it was evidenced by only 15% resistance.
Conclusion: Every treatment facility has microorganisms unique to it and these might change
with time. It is therefore of paramount importance to have an in-depth knowledge of the resident
organisms and their antibiotic sensitivity pattern so that infection related morbidity and mortality
will improved. The nature of microbial wound colonization and flora changes should be taken
into consideration in empirical antimicrobial therapy of burned patients.|
|Appears in:||Thesis - Medical Microbiology|
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