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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/2091

Title: Determination of the levels of essential and toxic metal constituents in cow's whol milk from selected sub-cities in addis ababa ethiopia
Authors: Dawd, Gashu
Advisors: Prof. Negussie Retta
Dr. Gulelate Dessie
Keywords: FA-AAS
Whole cow milk
Chromium
Nickel
Lead
Cadmium
Iron
Zinc
Copyright: Jun-2010
Date Added: 2-May-2012
Publisher: aau
Abstract: Whole milk samples were collected from 32 dairy farms found in Akaki-Kality, Bole, Kolfe, and Yeka sub-cities of Addis Ababa using prewashed polyethylene bottle. Whole milk samples were kept in an ice box and transported to EHNRI, Traditional and Modern Drug Research laboratory and kept in -20oC. The frozen samples were freeze dried in a freeze drying unit until a constant mass was achieved. The freeze dried samples were digested by wet ashing method using Conc.HNO3 and HClO4 in Kjeldahl apparatus. The digested were diluted by deionized water in to a volume of 50ml.The level of essential (iron, zinc, nickel and chromium) and toxic (lead and cadmium) metals in the samples was determined by FA-AAS. Statistical analysis was performed using SPPS version 17. The data were subjected to one way ANOVA using Duncan’s multiple range test. Contents of essential minerals were found to be in the normal range with other countries, while most of samples were below detection limit for chromium. Comparing the level of Pb and Cd in samples of the present with the acceptable limits, they were found to be beyond the limit. The average concentrations of the elements were Zn (4.923±0.277 mg/kg), Fe (1.213±0.077 mg/kg), Pb (0.998±0.251 mg/kg), Ni (0.784±0.054 mg/kg), Cd (0.100±0.006 mg/kg), Cr (0.036±0.004 mg/kg). Significant difference was not observed among the four sub cities with respect to iron, nickel, and cadmium content. Pb content of samples from Bole and Akaki-kality subcity was not significantly different, and also samples from Kolfe and Yeka subcities were significantly different with respect to their Pb content. The Zn content of samples from Bole, Kolfe, and Yeka were not significantly different. But Zn content of whole cow milk samples from Akaki-Kality were significantly different from Bole and Yeka but not with Kolfe (P = 0.05).
URI: http://hdl.handle.net/123456789/2091
Appears in:Thesis - Food Engineering

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