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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1971

Title: Chemometrically-Assisted Spectrophotometric Determ ination of Certain Anti-Tubercular Combinations
Authors: Yared, Mekuria
Advisors: Prof. Dr. Adel-Maaboud Ismail Mohammed,Dr. Ariaya Hymete
Keywords: Keywords: Chemometrics; Spectrophotometry; Rifampicin; Isoniazid; Pyrazinamide; Stability
Copyright: 2008
Date Added: 26-Jan-2009
Publisher: Addis Ababa University
Abstract: Abstract The UV absorption spectra of the rifampicin, isoniazid and pyrazinamide in the range of 200-400 nm, showed a considerable degree of spectral overlap. It has been reported that rifampicin and isoniazid are known to interact with each other in solid formulation milieu to yield isonicotinyl hydrazone. The use of chemometrics assisted spectrophtometric method is presented for the simultaneous determination of isoniazid and rifampicin tablets used in the treatment of tuberculosis. Resolution of binary mixtures of the drugs has been accomplished by using first derivative ratio, first derivative zero crossing. The multivariate methods, classical least squares (CLS) regression and principal components regression (PCR) has also been used for the determination of binary and ternary (in presence of pyrazinamide) mixtures. The amount of isoniazid and rifampicin in binary mixture were determined by measuring the first derivative zero-crossing wave lengths at 230 and 250.5 nm (zero-crossing wave length of rifampicin) and 320.5 and 360.5 nm (zero-crossing wave length of isoniazid) respectively. By using rifampicin as a divisor the amount of isoniazid in the same binary mixture were determined by measuring the first derivative ratio amplitudes at 222.5, 247.5, and 282.5 nm. Also by using isoniazid as divisor the amount of rifampicin were determined by measuring the first derivative ratio amplitudes at 322.5 and 327.5 nm. Although the components show an important degree of overlap, good recoveries were obtained with both the laboratory prepared mixtures and commercial tablets. No interference has been observed from the tablet excipients. The stability study indicated that the rate of degradation of rifampicin in the presence of isoniazid follows first order kinetics and the decreased amount is about 23.2% (RIF-INH in mixtures) and 30.9% (RIF-INH in formulations) through 150 minute at 60 oC.
Description: A Thesis Submitted to the School of Graduate Studies, Addis Ababa University in Partial Fulfilment of the Requirement for the Degree of Masters of Science in Pharmaceutical Analysis and Quality Assurance
URI: http://hdl.handle.net/123456789/1971
Appears in:Thesis - Pharmacology

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