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Addis Ababa University Libraries Electronic Thesis and Dissertations: AAU-ETD! >
School of Pharmacy >
Thesis - Pharmaceutical Analysis & Quality Assurance >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1941
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| Title: | CHEMOMETRICS ASSISTED UV- SPECTROPHOTOMETRIC DETERMINATION OF TOPICAL BINARY MIXTURES CONTAINING BENZOIC ACID, SALICYLIC ACID OR RESORCINOL |
| Authors: | Mulualem, Kassa |
| Advisors: | Prof.Dr.Abdel-Maaboud I Mohamed |
| Keywords: | chemometrics
derivative spectrophotometry
CLS
PCR
pharmaceuticals
white field ointment
luna soap
dorin emulsion |
| Copyright: | 2008 |
| Date Added: | 20-Jan-2009 |
| Publisher: | Addis Ababa University |
| Abstract: | Chemometrics-assisted spectrophotometric methods were developed for simultaneous
determinations of binary mixtures of (1). benzoic acid and salicylic acid, and (2). resorcinol and
salicylic acid mixture. The commercial forms were marketed in Ethiopia and Egypt, such as
whitefield ointment, luna soap, and dorin emulsion. The uv absorption spectra of the studied
compounds in the range of 200-400 nm, showed considerable degrees of spectral overlapping
(between 87.6% for benzoic acid and salicylic acid mixture, and 96.2% for salicylic acid and
resorcinol mixture). The resolution of the mixtures have been successfully accomplished by
using four techniques namely,1D (first derivative), 1D ratio, CLS (classical least square) and PCR
(principal component regression). Variable results were obtained in the different cases as
explained in the core of this thesis.
Validation parameters such as calibration range, LOD, LOQ, precision and accuracy parameters
(Between F- and t-values respectively) were calculated and a comparison between all the
methods and some official methods were done.
The results showed that the PCR technique was the most suitable for analysis of such binary
mixtures or the commercial formulations, more than the other techniques and agrees well with
those results obtained from the official method. |
| Description: | A thesis Submitted to the School of Graduate Studies of Addis Ababa University in
partial fulfilment of the requirement for the Degree of Master of Science in
Pharmaceutical Analysis and Quality Assurance |
| URI: | http://hdl.handle.net/123456789/1941 |
| Appears in: | Thesis - Pharmaceutical Analysis & Quality Assurance
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