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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1937

Authors: Behailu, Urgessa
Advisors: Prof. Dr. Abdel-Maaboud Ismail Mohamed
Copyright: 2008
Date Added: 20-Jan-2009
Publisher: Addis Ababa University
Abstract: A simple, accurate and precise high-performance thin-layer chromatographic (HPTLC) method has been developed for the determination of vitamin B1, B6 and B12 in tablet and injection. Using methanol: ammonium acetate (5M) (10:1; v/v) as mobile phase and HPTLC plates (0.2mm thickness) precoated with 60F254 silica gel on glass plate as stationary phase. Quantification was carried out densitometrically using a UV detector at 255, 290 and 361nm. The retardation factors (Rf) of B1, B6 and B12 were 0.20 ± 0.02, 0.40 ± 0.02 and 0.68 ± 0.02 respectively. Calibration curves were polynomial in the range 250-2050 ng/μl for B1 and B6, 50-1050 ng/μl for B12. The method has been robust for small variation with mobile phase composition, amount of mobile phase and time variation before chromatographic development and scanning. The stability of sample solution was studied over eight hours and no additional spot was observed on the plate. As a result no evidence was observed for the degradation of the product. In addition the proposed method has been compared statistically (t-test and Ftest) with the classical method (HPLC) and there was no difference in terms of accuracy of the dosage analysis between the two methods. The analysis result of both synthetic mixture and pharmaceutical samples showed the quantitative determination of each component accurately in the presence of the other components and back ground interference. Assay of vitamin B1, B6 and B12 were 99.10 ± 1.37 %, 99.68 ± 1.50 % and 99.42 ± 1.26 % for vitamin B1, B6 and B12 injection, 99.44 ± 1.30 % and 99.95 ± 1.49 % for vitamin B1 and B6 tablet respectively.
Description: A Thesis Presented to the School of Graduate Studies of Addis Ababa University in Partial Fulfillment of the Requirements of the Degree of Master of Science in Pharmaceutical Analysis and Quality Assurance
URI: http://hdl.handle.net/123456789/1937
Appears in:Thesis - Pharmaceutical Analysis & Quality Assurance

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