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Addis Ababa University Libraries Electronic Thesis and Dissertations: AAU-ETD! >
School of Pharmacy >
Thesis - Pharmaceutical Analysis & Quality Assurance >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/123456789/1937
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| Title: | HPTLHPTLC-DENSITOMETRIC DETERMINATION OF SOME WATER SOLUBLE VITAMINS PRESENT IN PHARMACEUTICALS |
| Authors: | Behailu, Urgessa |
| Advisors: | Prof. Dr. Abdel-Maaboud Ismail Mohamed |
| Copyright: | 2008 |
| Date Added: | 20-Jan-2009 |
| Publisher: | Addis Ababa University |
| Abstract: | A simple, accurate and precise high-performance thin-layer chromatographic (HPTLC)
method has been developed for the determination of vitamin B1, B6 and B12 in tablet and
injection. Using methanol: ammonium acetate (5M) (10:1; v/v) as mobile phase and
HPTLC plates (0.2mm thickness) precoated with 60F254 silica gel on glass plate as
stationary phase. Quantification was carried out densitometrically using a UV detector at
255, 290 and 361nm. The retardation factors (Rf) of B1, B6 and B12 were 0.20 ± 0.02, 0.40
± 0.02 and 0.68 ± 0.02 respectively. Calibration curves were polynomial in the range
250-2050 ng/μl for B1 and B6, 50-1050 ng/μl for B12.
The method has been robust for small variation with mobile phase composition, amount
of mobile phase and time variation before chromatographic development and scanning.
The stability of sample solution was studied over eight hours and no additional spot was
observed on the plate. As a result no evidence was observed for the degradation of the
product. In addition the proposed method has been compared statistically (t-test and Ftest)
with the classical method (HPLC) and there was no difference in terms of accuracy
of the dosage analysis between the two methods.
The analysis result of both synthetic mixture and pharmaceutical samples showed the
quantitative determination of each component accurately in the presence of the other
components and back ground interference.
Assay of vitamin B1, B6 and B12 were 99.10 ± 1.37 %, 99.68 ± 1.50 % and 99.42 ± 1.26
% for vitamin B1, B6 and B12 injection, 99.44 ± 1.30 % and 99.95 ± 1.49 % for vitamin
B1 and B6 tablet respectively. |
| Description: | A Thesis Presented to the School of Graduate Studies of Addis Ababa
University in Partial Fulfillment of the Requirements of the Degree of
Master of Science in Pharmaceutical Analysis and Quality Assurance |
| URI: | http://hdl.handle.net/123456789/1937 |
| Appears in: | Thesis - Pharmaceutical Analysis & Quality Assurance
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