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Title: Nodulation Pattern and Biodiversity of Endosymbionts of Some Woody Legumes of Ethiopia
Authors: Diriba, Temesgen
Date Added: 21-Sep-2007
Abstract: A collection of 21 root nodule bacteria were isolated from some woody legumes that belong to 8 genera from various regions of Ethiopia. The isolates were presumptively tested to check contaminations followed by morphological and physiological characterizations, and numerical analysis of phenotypic data using a computer cluster analysis applying UPGMA (NT-SYS version 2.1). Isolates of Erythrina brucei and a species of Crotalaria, Indigofera and Tephrosia were selected as representatives and cross-inoculated on Phaseolus vulgaris and Vigna unguiculata for authentication and symbiotic effectiveness evaluation .The isolates showed a wide range of morphological and physiological characterstics. Most of the isolates were found to be slow growing, NaCl and streptomycin sulfate sensitive but able to utilize a wide range of carbohydrates, and grow over a wider temperature and pH ranges. The analysis of phenotypic data revealed that at a boundary level of 64.8% average similarity, all the isolates were grouped in to 3 clusters while at 92.2% the isolates were further grouped in to 5 clusters except 8 isolates. All the four crossinoculated representative isolates elicited nodulation on Phaseolus vulgaris but one of them failed to nodulate Vigna unguiculata, which might be due to genetic incompatibility. An isolate of Crotalaria species showed the highest symbiotic effectiveness with both Vigna unguiculata and Phaseolus vulgaris suggesting that the isolate is worthy of further field testing and of great potential application in improving the yield of these grain legumes.
Description: A Thesis Submitted to the School of Graduate studies Department of Biology In partial fulfillment of the requirements of the degree of Master’s of Science in Biology, Applied Microbiology
URI: http://hdl.handle.net/123456789/129
Appears in:Thesis - Biology

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