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Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/1209

Title: ANTISPASMODIC, ANTIDIARRHEAL and LD50 DETERMINATION of Syzygium guineense in ANIMAL MODELS
Authors: BIZUAYEHU, NIGATU
Advisors: Professor Eyasu Makonnen
Copyright: 2004
Date Added: 23-May-2008
Publisher: Addis Ababa University
Abstract: Aqueous and hydroalcoholic extracts of dried twigs, stem barks and fruits of Syzygium guineense were tested on contraction of isolated guinea pig ileum (GPI) in vitro; intestinal transit and castor oil-induced diarrhea test in mice in vivo. Different concentrations of each extract of the plant were used in the presence of agonist controls:- ACh and histamine (in GPI) as contraction stimulators in vitro, atropine and dexchlorpheniramine were used in GPI and atropine and loperamide in the intestinal transit and antidiarrheal test, respectively, as positive control. The leaf aqueous (Laq) and hydroalcoholic (Lm) extracts, exhibited significant dose-dependent reductions in ACh and histamine-induced commulative contractions (P< 0.001). The spontaneous agonist-induced contractions of GPI were greatly reduced by Laq and Lm at maximal dosages suggesting the spasmolytic property of the crude extracts. All the extracts except Laq and Lm (with dose of 100 and 200μg/ml) were less potent than atropine (6.66x10-9 M) and dexchlorpheniramine (1.3x10-9 M) in the experiment of GPI. Most doses of the extracts (i.e Laq, Lm, SBaq) showed antitransit activity in the intestine of mice and Laq (200mg/g) showed comparable effects with that of atropine. All the six extracts (i.e. Laq, Lm, SBaq, SBm, Faq and Fm) showed significant antidiarrheal activity against castor oil-induced diarrhea. The LD50 of Laq, Lm, Sbaq, SBm, and Fm were 14.10, 2.91, 5.12, 8.77 and >10.0g/kg respectively. The present study suggested that the plants studied possess spasmolytic, antitransit and antidiarrheal properties due to the presence of flavonoid, tannin or cumarins shown to be present in some extracts. The results also support the traditional folk use of the twigs and stem bark parts of the plants for stomach pains, intestinal cramps and diarrhea. Further study should be pursued in order to find the exact mechanism of action and to characterize major secondary metabolites responsible for the activity observed.
Description: A thesis submitted to School of Graduate studies in partial fulfillment of the degree of Master of Science in Pharmacology, Department of Pharmacology, Faculty of Medicine, AAU.
URI: http://hdl.handle.net/123456789/1209
Appears in:Thesis - Pharmacology

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